Evaluated: Boncompain et al (2012) Synchronization of secretory protein traffic in populations of cells.

I evaluated this for F1000 (yes, I’ve been busy!). Looks like a really useful system for many, especially given its adaptability.

G Boncompain, S Divoux, N Gareil, H de Forges, A Lescure, L Latreche, V Mercanti, F Jollivet, G Raposo and F Perez  (2012) Synchronization of secretory protein traffic in populations of cells. Nature Methods 2012 9 PMID: 22406856 DOI: 10.1038/nmeth.1928

This paper presents an exciting and widely modifiable system to study organelle dynamics and secretory protein traffic in a controlled manner. The retention using selective hooks (RUSH) system is based on controlled retention of proteins in the endoplasmic reticulum (ER) allowing one to exploit any number of targets through genetic engineering of relevant reporters. There are many advantages over other systems that have been highlighted by the other evaluations. The system presents some great options for both image-based and biochemical assays. I entirely agree with their comments and look forward to seeing this system in wide use (I note there are no restrictions on academic use).

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